What is the clinical evidence from this study?

Study design: Other. Population: Diabetic retinopathy (experimental). Intervention: Valsartan or PD123319 vs. Untreated diabetic or angiotensin II-infused rats. Primary outcome: Retinal VEGF expression.

What question did this study set out to answer?

To determine whether angiotensin type 2 receptor blockade attenuates retinal vascular endothelial growth factor (VEGF) expression in diabetic and angiotensin II-infused rat models.

January 13, 2004Hypertension

Retinal Expression of Vascular Endothelial Growth Factor Is Mediated by Angiotensin Type 1 and Type 2 Receptors

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Key result

Treatment with either the AT1 receptor antagonist valsartan or the AT2 receptor antagonist PD123319 attenuated retinal VEGF expression in both diabetic and angiotensin II-infused rats.

Why the study?

Does AT1 or AT2 receptor blockade attenuate retinal VEGF expression in diabetic or angiotensin II-infused rats?

Population

Experimental diabetic rats induced by streptozotocin injection, and rats infused with angiotensin II

Comparison

Valsartan or PD123319 administered for 4 weeks… vs Untreated diabetic rats or untreated angiotensin…

Design

Preclinical

Follow-up

2 to 4 weeks

Authors

XZXiaoli ZhangShenyang Pharmaceutical University MLMarkus LassilaUniversity of Helsinki MCMark E. CooperUniversité Claude Bernard Lyon 1

Discussion

Member takes

Implication

Supports dual AT1/AT2 involvement in experimental retinal VEGF upregulation; leaves open clinical translation to diabetic retinopathy.

Key Points

To determine whether angiotensin type 2 receptor blockade attenuates retinal vascular endothelial growth factor (VEGF) expression in diabetic and angiotensin II-infused rat models.
Treated streptozotocin-induced diabetic rats with the AT1 receptor antagonist valsartan or the AT2 receptor antagonist PD123319 for 4 weeks.
Administered valsartan or PD123319 for 2 weeks to non-diabetic rats receiving continuous angiotensin II infusions.
Quantified retinal VEGF gene and protein expression using real-time RT-PCR and immunostaining.
Retinal gene and protein expression of VEGF increased substantially in both diabetic rats and angiotensin II-infused rats.
Blockade of either AT1 receptors with valsartan or AT2 receptors with PD123319 attenuated retinal VEGF overexpression across both experimental models.

Structured PICO

Does AT1 or AT2 receptor blockade attenuate retinal VEGF expression in diabetic or angiotensin II-infused rats?

Population

Experimental diabetic rats and angiotensin II-infused rats evaluated for retinal VEGF expression after treatment with angiotensin receptor antagonists.

Intervention

Valsartan (AT1 receptor antagonist) or PD123319 (AT2 receptor antagonist) administered for 4 weeks (diabetic rats) or 2 weeks (angiotensin II infused rats)

Comparator

Untreated diabetic rats or untreated angiotensin II infused rats

Outcome

Retinal VEGF gene and protein expression assessed by real-time reverse transcription-polymerase chain reaction and immunostainingsurrogate

Both AT1 and AT2 receptors mediate angiotensin II-induced VEGF expression in the retina, suggesting a role for both receptor subtypes in the pathogenesis of diabetic retinopathy.

Cite This Study

Zhang et al. (2004) studied Diabetic retinopathy (experimental). Valsartan or PD123319 vs. Untreated diabetic or angiotensin II-infused rats was evaluated on Retinal VEGF expression. Treatment with either the AT1 receptor antagonist valsartan or the AT2 receptor antagonist PD123319 attenuated retinal VEGF expression in both diabetic and angiotensin II-infused rats.

synapsesocial.com/papers/6a9388d81df2c3eba81f4c2f https://doi.org/10.1161/01.hyp.0000113628.94574.0f

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Retinal Expression of Vascular Endothelial Growth Factor Is Mediated by Angiotensin Type 1 and Type 2 Receptors

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Retinal Expression of Vascular Endothelial Growth Factor Is Mediated by Angiotensin Type 1 and Type 2 Receptors

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider