What question did this study set out to answer?

To characterize the electrical properties of sinus node pacemaker cells and evaluate an in vitro perfused canine right atrium preparation for microelectrode impalement studies.

July 1, 1976Circulation Research

Spontaneous action potentials of cells in the canine sinus node.

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Population

Right atria excised from 20 young dogs (15 +/- 5 weeks old)

Design

Preclinical

Follow-up

at least 6 hours

Authors

WWW T WoodsAtrium Health Wake Forest Baptist FUFerdinand UrthalerUniversity of Alabama at Birmingham TJThomas N. JamesTulane University

Discussion

Member takes

Implication

Supports sinus node model development in vitro; leaves open human translation pending further validation.

Key Points

To characterize the electrical properties of sinus node pacemaker cells and evaluate an in vitro perfused canine right atrium preparation for microelectrode impalement studies.
Excised right atria from 20 young dogs (aged 15 ± 5 weeks) were maintained in vitro and perfused with Krebs-Ringer bicarbonate solution via the sinus node artery.
Intracellular microelectrode recordings were performed in tissue between the superior vena cava and right atrium, verified histologically as the midportion of the sinus node.
Excised atria maintained spontaneous contractions for at least 6 hours at a stable rate of 127 beats per minute.
Sinus node pacemaker cells displayed a maximum diastolic potential of -56 ± 7 mV, action potential amplitude of 56 ± 8 mV, overshoot of 0 ± 2 mV, and smooth phase 4 to phase 0 transitions.

Structured PICO

Population

Right atria excised from 20 young dogs (15 +/- 5 weeks old)

Intervention

In vitro perfusion with Krebs-Ringer bicarbonate solution through the sinus node artery and intracellular microelectrode impalement

Outcome

Transmembrane potentials of pacemaker cells (maximum diastolic potential, action potential amplitude, overshoot) and spontaneous contraction ratesurrogate

The excised perfused canine right atrium is a viable and stable in vitro model for studying sinus node electrophysiology using intracellular microelectrodes.

Cite This Study

Woods et al. (1976) studied this question.

synapsesocial.com/papers/6a73a7ea7af34f94aaa736db https://doi.org/10.1161/01.res.39.1.76

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Spontaneous action potentials of cells in the canine sinus node.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Spontaneous action potentials of cells in the canine sinus node.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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