April 15, 2014PLoS ONEOpen Access

The Muscle Ankyrin Repeat Proteins CARP, Ankrd2, and DARP Are Not Essential for Normal Cardiac Development and Function at Basal Conditions and in Response to Pressure Overload

View Full Paper

Ask AI

Bookmark

Population

Wildtype (WT) and knockout (KO) mice for CARP, Ankrd2, and DARP (single, double, and triple MARP KO mice)

Comparison

Genetic ablation of CARP, Ankrd2, and DARP and… vs Wildtype mice with the same genetic background…

Design

Preclinical

Follow-up

Up to 17 months of age; 14 days after TAC

Authors

MBMarie‐Louise BangIRCCS Humanitas Research Hospital YGYusu GuHeart Failure & Transplant NDNancy D. DaltonCardiac Imaging

Discussion

Member takes

Implication

MARP proteins dispensable for pressure-overload response in mice; hypothesis-generating for their role in human heart failure.

Structured PICO

Population

Wildtype (WT) and knockout (KO) mice for CARP, Ankrd2, and DARP (single, double, and triple MARP KO mice)

Intervention

Genetic ablation of CARP, Ankrd2, and DARP (triple knockout) and transverse aortic constriction (TAC) to induce mechanical pressure overload

Comparator

Wildtype (WT) mice with the same genetic background, undergoing sham or TAC

Outcome

Cardiac development, morphology, and function at basal conditions and in response to mechanical pressure overload (assessed by echocardiography and hemodynamics)surrogate

The muscle ankyrin repeat proteins CARP, Ankrd2, and DARP are not essential for normal cardiac development and function at basal conditions or in response to mechanical pressure overload in vivo.

Limitations

Constitutive KO models may allow compensatory mechanisms to counteract the effect of the absence of MARPs
Lack of a cardiac phenotype after 14 days of TAC does not exclude a phenotype after long-term TAC or in response to other types of stress conditions

Cite This Study

Bang et al. (2014) studied this question.

synapsesocial.com/papers/6a73dbd35e45dc7750a8be01 https://doi.org/10.1371/journal.pone.0093638

View Full Paper

Ask AI

Bookmark

The Muscle Ankyrin Repeat Proteins CARP, Ankrd2, and DARP Are Not Essential for Normal Cardiac Development and Function at Basal Conditions and in Response to Pressure Overload

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

The Muscle Ankyrin Repeat Proteins CARP, Ankrd2, and DARP Are Not Essential for Normal Cardiac Development and Function at Basal Conditions and in Response to Pressure Overload

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

Also Consider

Also Consider