April 29, 1997Proceedings of the National Academy of SciencesOpen Access

Ultrarapid, highly efficient viral gene transfer to the heart

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Why the study?

Does optimized adenovirus-mediated gene transfer improve transduction efficiency in rabbit ventricular myocytes and intact hearts?

Population

Cultured rabbit ventricular myocytes and intact rabbit hearts

Design

Preclinical

Authors

JDJ. Kevin DonahueUniversity of Maryland, Baltimore KKKohei KikkawaThe University of Tokyo DJDavid C. JohnsAmgen (United States)

Discussion

Member takes

Implication

Should not change clinical practice; leaves open translation of optimized ex vivo transduction to in vivo or human hearts.

Structured PICO

Does optimized adenovirus-mediated gene transfer improve transduction efficiency in rabbit ventricular myocytes and intact hearts?

Population

Cultured rabbit ventricular myocytes and intact rabbit hearts

Intervention

Adenovirus-mediated cardiac gene transfer (via intracoronary perfusion for intact hearts)

Outcome

Percentage of infected cells expressing the reporter gene beta-galactosidasesurrogate

Optimized adenovirus-mediated gene delivery via intracoronary perfusion achieves nearly 100% transduction of intact rabbit hearts ex vivo, providing a highly efficient method for cardiac gene transfer.

Cite This Study

Donahue et al. (1997) studied this question.

synapsesocial.com/papers/6a73ff495e45dc7750a8ca58 https://doi.org/10.1073/pnas.94.9.4664

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Ask AI

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Ultrarapid, highly efficient viral gene transfer to the heart

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Ultrarapid, highly efficient viral gene transfer to the heart

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider