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February 1, 1985Circulation

Inhibition by diphosphonate compounds of calcification of porcine bioprosthetic heart valve cusps implanted subcutaneously in rats.

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Why the study?

Do diphosphonate compounds inhibit calcification of subcutaneously implanted porcine bioprosthetic heart valve cusps in rats?

Population

3-week-old male rats with subcutaneously implanted glutaraldehyde cross-linked porcine aortic valve cusps

Comparison

Diphosphonate compounds vs Control valves (no diphosphonate treatment)

Design

Preclinical

Follow-up

Up to 21 days (or 35 days for prolonged effect assessment)

Authors

RLRobert J. LevyMHMarguerite HawleyFSFrederick J. Schoen

Discussion

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Overview

Does not support clinical use in bioprosthetic valves; leaves open translation from rat models to humans.

Structured PICO

Do diphosphonate compounds inhibit calcification of subcutaneously implanted porcine bioprosthetic heart valve cusps in rats?

P
Population
3-week-old male rats with subcutaneously implanted glutaraldehyde cross-linked porcine aortic valve cusps
I
Intervention
Diphosphonate compounds (EHDP via daily subcutaneous injection 25 mg/kg/24 hr for 21 days, local administration by osmotic pump 5 mg/kg/24 hr for 14 days, or preincubation in aminopropanehydroxydiphosphonate for 24 hr before 21 day implantation)
C
Comparator
Control valves (no diphosphonate treatment)
O
Outcome
Calcification of bioprosthetic valve cusps (measured as BC Ca++)surrogate

Diphosphonates inhibit calcification of bioprosthetic heart valves in a rat model, and adverse systemic effects can be avoided by local administration or preincubation.

Cite This Study

Levy et al. (1985) studied this question.

synapsesocial.com/papers/6a742dfebd21f773de3920fdhttps://doi.org/10.1161/01.cir.71.2.349
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Also Consider

Synapse has enriched 3 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Five to Eight-Year Follow-up of Patients Undergoing Porcine Heart-Valve Replacement1981 · 166 citations
  2. 2Atherosclerosis: Prevention by Agents Not Affecting Abnormal Levels of Blood Lipids1981 · 184 citations
  3. 3The Influence of Two Diphosphonates on Calcium Metabolism in the Rat1972 · 202 citations