Key result
A two-step PEGDA hydrogel surface-coating method for bioprosthetic valve models reduced protein adhesion to 2% compared to 20% on uncoated samples without significantly affecting mechanical properties.
Why the study?
Bioprosthetic heart valves face calcification and failure, but shielding them with hydrogels using traditional light-based polymerization is impeded by tissue opacity and topography.
Absolute Event Rate: 2% vs 20%
A novel two-step PEGDA coating method successfully creates a thin, continuous layer on bioprosthetic valve models that significantly reduces protein adhesion, potentially mitigating calcification and failure.
Two-step hydrogel coating may shield bioprosthetic valves from calcification; leaves open clinical translation pending in vivo testing.
Bioprosthetic heart valve implants are beset by calcification and failure due to the interactions between the body and the transplant. Hydrogels can be used as biological blank slates that may help to shield implants from these interactions; however, traditional light-based hydrogel polymerization is impeded by tissue opacity and topography. Therefore, new methods must be created to bind hydrogel to implant tissues. To address these complications, a two-step surface-coating method for bioprosthetic valves was developed. A previously developed bioprosthetic valve model (VM) was used to investigate and optimize the coating method. Generally, this coating is achieved by first reacting surface amine groups with an NHS-PEG-acrylate while also allowing glucose to absorb into the bulk. Then, glucose oxidase, poly(ethylene glycol) diacrylate (PEGDA), and iron ions are added to the system to initiate free-radical polymerization that bonds the PEGDA hydrogel to the acrylates sites on the surface. Results showed a thin (∼8 μm), continuous coating on VM samples that is capable of repelling protein adhesion (2% surface fouling versus 20% on uncoated samples) and does not significantly affect the surface mechanical properties. Based on this success, the coating method was translated to glutaraldehyde-fixed valve tissue samples. Results showed noncontinuous but evident coating on the surface, which was further improved by adjusting the coating solution. These results demonstrate the feasibility of the proposed two-step surface coating method for modifying the surface of bioprosthetic valve replacements.
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Roseen et al. (2020) studied Bioprosthetic heart valve implants. Two-step surface-coating method using PEGDA hydrogel vs. Uncoated samples was evaluated on Protein adhesion (surface fouling). A two-step PEGDA hydrogel surface-coating method for bioprosthetic valve models reduced protein adhesion to 2% compared to 20% on uncoated samples without significantly affecting mechanical properties.
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