September 2, 1997Circulation
Amiodarone Inhibits Production of Tumor Necrosis Factor-α by Human Mononuclear Cells
Why the study?
Does amiodarone inhibit the production of cytokines (TNF-alpha, IL-1beta, IL-6) in human mononuclear cells compared to other antiarrhythmic agents?
Population
Human peripheral blood mononuclear cells (PBMC) obtained from healthy volunteers
Comparison
Amiodarone cultured with PBMC in the presence of… vs Quinidine, disopyramide, and lidocaine cultured…
Design
Preclinical
Follow-up
24 hours
Authors
Discussion
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Implication
Amiodarone may modulate cytokines in animal models; leaves open immunomodulatory role in human heart failure.
Structured PICO
Does amiodarone inhibit the production of cytokines (TNF-alpha, IL-1beta, IL-6) in human mononuclear cells compared to other antiarrhythmic agents?
P
Population
Human peripheral blood mononuclear cells (PBMC) obtained from healthy volunteers
I
Intervention
Amiodarone (0.1, 1, and 10 micromol/L) cultured with PBMC in the presence of lipopolysaccharide for 24 hours
C
Comparator
Quinidine, disopyramide, and lidocaine (0.1, 1, and 10 micromol/L) cultured with PBMC in the presence of lipopolysaccharide for 24 hours
O
Outcome
Production of TNF-alpha, IL-1beta, and IL-6 measured in culture supernatantssurrogate
Amiodarone inhibits the production of TNF-alpha and IL-6 in human mononuclear cells, suggesting an immunomodulatory mechanism that may contribute to its beneficial effects in heart failure.
Cite This Study
Matsumori et al. (1997) studied this question.