What question did this study set out to answer?

To measure, activate, and biochemically characterize active and inactive renin forms across cat plasma, peritoneal dialysate, and kidney tissues.

January 1, 1987Clinical and Experimental Hypertension

Active and Inactive Renin in the Cat

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Population

Pentobarbitone-anesthetized cats

Design

Preclinical

Authors

IMI. M. McKenzieLurie Children's Hospital DHDiana L. HeimanHartford Hospital JMJ. K. McKenzieUniversity of Florida

Discussion

Member takes

Implication

Animal data on feline renin isoforms should not guide human therapy; leaves open whether inactive renin modulates inhibitor response in vivo.

Key Points

To measure, activate, and biochemically characterize active and inactive renin forms across cat plasma, peritoneal dialysate, and kidney tissues.
Collected blood, kidneys, and peritoneal dialysate fluid from pentobarbitone-anesthetized cats.
Activated inactive renin using acid (pH 4.0) or trypsin (2 mg/ml at 4 °C for 10 minutes) and separated fractions via Cibacron-Blue affinity chromatography.
Characterized pH optima, apparent molecular weights, and inhibition kinetics with renin inhibitor H77.
Baseline feline plasma contained active renin at 1.8 +/- 0.4 pmoles AI/ml/hr and inactive renin at 2.3 +/- 0.5 pmoles AI/ml/hr, with trypsin activation yielding substantially higher activity than acid activation.
Cibacron-Blue affinity chromatography isolated 30% active renin in the unbound fraction and 70% inactive renin in the bound peak.
Active plasma renin (MW 33,500), kidney renin (MW 36,000), and activated inactive dialysate renin (MW 39,000–40,000) all displayed identical inhibition by H77 with an IC50 of 0.3 microM.

Structured PICO

Population

Pentobarbitone-anesthetized cats

Intervention

Biochemical analysis of active and inactive renin (including trypsin activation, cibacron-blue affinity chromatography, and inhibition by H77)

Outcome

Levels and biochemical properties (molecular weight, pH optimum, inhibition by H77) of active and inactive renin in plasma, peritoneal dialysate, and kidneysurrogate

This preclinical study characterizes active and inactive renin in cats, demonstrating that inactive renin is abundant, can be activated by trypsin, and shares similar inhibition properties with active renin.

Cite This Study

McKenzie et al. (1987) studied this question.

synapsesocial.com/papers/6a771fc3d3a6aa87c6bd9522 https://doi.org/10.3109/10641968709159007

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Implication

Animal data on feline renin isoforms should not guide human therapy; leaves open whether inactive renin modulates inhibitor response in vivo.

Active and Inactive Renin in the Cat

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Active and Inactive Renin in the Cat

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider