Key result
A minimally invasive surgical procedure for inducing myocardial infarction in mice was established, achieving an infarction volume of 15-20% in chronic models with a mortality rate of 10%.
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This minimally invasive mouse model of myocardial infarction reduces surgical trauma and mortality while maintaining reproducible infarct sizes, supporting the 3R principles in preclinical cardiovascular research.
Myocardial infarction still remains the main cause of death in western countries, despite considerable progress in the stent development area in the last decades. For clarification of the underlying mechanisms and the development of new therapeutic strategies, the availability of valid animal models are mandatory. Since we need new insights into pathomechanisms of cardiovascular diseases under in vivo conditions to combat myocardial infarction, the validity of the animal model is a crucial aspect. However, protection of animals are highly relevant in this context. Therefore, we establish a minimally invasive and simple model of myocardial infarction in mice, which assures a high reproducibility and survival rate of animals. Thus, this models fulfils the requirements of the 3R principle (Replacement, Refinement and Reduction) for animal experiments and assure the scientific information needed for further developing of therapeutical strategies for cardiovascular diseases.
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Curaj et al. (2015) studied Myocardial infarction (animal model). Minimally invasive surgical procedure (LAD ligation) was evaluated on Infarction size and mortality rate (methodological demonstration). A minimally invasive surgical procedure for inducing myocardial infarction in mice was established, achieving an infarction volume of 15-20% in chronic models with a mortality rate of 10%.
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