What question did this study set out to answer?

The aim is to explore the heterogeneity of angiotensin II receptor subtypes in specific rat brain regions.

January 1, 1991Journal of Neurochemistry

Quantitative Autoradiography Reveals Different Angiotensin II Receptor Subtypes in Selected Rat Brain Nuclei

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Population

Rat brain nuclei

Design

Preclinical

Authors

KTKeisuke TsutsumiHiroshima University JSJuan M. SaavedraGeneral / Preventive / Lipids

Discussion

Member takes

Implication

Distinguishes rat brain AT1/AT2 distributions; hypothesis-generating and should not yet change practice.

Key Points

The aim is to explore the heterogeneity of angiotensin II receptor subtypes in specific rat brain regions.
Utilized quantitative autoradiography after incubating with 125I-Sar1-angiotensin II.
Displacement studies performed with angiotensin II antagonists CGP 42112 A and DuP-753.
Sensitivity to dithiothreitol was examined to differentiate receptor types.
Angiotensin II type-1 receptors characterized in cardiovascular regions with IC50 of 1 x 10(-7) M for DuP-753.
Angiotensin II type-2 receptors found in the inferior olive, displaced by CGP 42112 A with IC50 of 1 x 10(-9) M.
Type-1 receptors sensitive to 5 mM dithiothreitol, while type-2 receptors remain insensitive.

Structured PICO

Population

Rat brain nuclei (subfornical organ, nucleus of the solitary tract, paraventricular nucleus, area postrema, inferior olive)

Intervention

Incubation with 125I-Sar1-angiotensin II and displacement with angiotensin II antagonists CGP 42112 A and DuP-753, and dithiothreitol

Outcome

Angiotensin II receptor subtype identification (displacement IC50 and dithiothreitol sensitivity)surrogate

This preclinical study demonstrates the heterogeneity of angiotensin II receptors in the rat brain, identifying distinct AT1 and AT2 subtypes in different nuclei.

Cite This Study

Tsutsumi et al. (1991) studied this question.

synapsesocial.com/papers/6a779319137c7dcc81bde9db https://doi.org/10.1111/j.1471-4159.1991.tb02602.x

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Quantitative Autoradiography Reveals Different Angiotensin II Receptor Subtypes in Selected Rat Brain Nuclei

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Quantitative Autoradiography Reveals Different Angiotensin II Receptor Subtypes in Selected Rat Brain Nuclei

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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