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November 1, 1965The Journal of Experimental MedicineOpen Access

Studies of Adenovirus Sv40 Hybrid Viruses

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Population

African green monkey kidney or human embryonic kidney tissue culture cells infected with adenovirus SV40…

Comparison

50 degrees C heat inactivation… vs Adenovirus and SV40 virus grown as a mixed…

Design

Preclinical

Authors

WRWallace P. RoweNew York State Department of HealthSBStephen G. BaumAlbert Einstein College of MedicineWPWendell E. PughNational Institutes of Health

Discussion

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Implication

Confirms hybrid nature of E46(+) preparations in animal models; leaves open relevance to human viral interactions.

Structured PICO

P
Population
African green monkey kidney (AGMK) or human embryonic kidney tissue culture (HEK) cells infected with adenovirus SV40 hybrid viruses (E46(+))
I
Intervention
50 degrees C heat inactivation, ultracentrifugation, equilibrium density gradient centrifugation, and pretreatment with 5-fluorouracil desoxyriboside (FUDR)
C
Comparator
Adenovirus and SV40 virus grown as a mixed infection (for physical procedures)
O
Outcome
Titers of adenovirus and SV40 genetic carriers (quantitated by percentage of cells showing neoantigens stainable with fluorescent antibody)surrogate

The study demonstrates that SV40 neoantigen induction cannot be physically dissociated from adenovirus in E46(+) hybrid preparations, confirming their hybrid nature.

Cite This Study

Rowe et al. (1965) studied this question.

synapsesocial.com/papers/6a77cbc898f24dbf64bd9d0bhttps://doi.org/10.1084/jem.122.5.943
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