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January 1, 2001Journal of Biological ChemistryOpen Access

A Kinetic Simulation Model That Describes Catalysis and Regulation in Nitric-oxide Synthase

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Population

neuronal NO synthase (nNOS) and mutants W409F and W409Y (computational kinetic model)

Design

Preclinical

Authors

JSJérôme SantoliniSASubrata AdakCCChristine M. L. Curran

Discussion

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Overview

No immediate clinical implications; extends computational tools for nNOS research while leaving in vivo validation open.

Structured PICO

P
Population
neuronal NO synthase (nNOS) and mutants W409F and W409Y (computational kinetic model)
I
Intervention
10-step kinetic simulation model
O
Outcome
Pre-steady and steady-state features of nNOS catalysis (NADPH consumption, NO production, heme-NO complex buildup)

A novel 10-step kinetic model accurately simulates nNOS catalysis and regulation, providing a tool for understanding its catalytic behavior.

Cite This Study

Santolini et al. (2001) studied this question.

synapsesocial.com/papers/6a77edddd2fa2470761f3275https://doi.org/10.1074/jbc.m006858200
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Analysis of Neuronal NO Synthase under Single-Turnover Conditions:  Conversion of<i>N</i><sup>ω</sup>-Hydroxyarginine to Nitric Oxide and Citrulline1997 · 59 citations
  2. 2Nitric Oxide Synthases: Properties and Catalytic Mechanism1995 · 1,296 citations
  3. 3Stopped-Flow Analysis of CO and NO Binding to Inducible Nitric Oxide Synthase1998 · 103 citations
  4. 4Nitric Oxide-Generated P420 Nitric Oxide Synthase:  Characterization and Roles for Tetrahydrobiopterin and Substrate in Protecting against or Reversing the P420 Conversion1999 · 43 citations
  5. 5Stopped-Flow Analysis of Substrate Binding to Neuronal Nitric Oxide Synthase1999 · 24 citations