Key result
Periostin treatment effectively repaired isoproterenol-induced damage in human bone marrow mesenchymal stem cell-derived cardiomyocytes, evidenced by increased cardiac markers and decreased LDH.
Why the study?
Assessing new therapeutic drugs for heart disease is challenging, driving the need for feasible cellular models to evaluate cardiac damage and repair instead of animal models.
May enable in vitro cardiac drug testing; leaves open clinical translation of stem cell therapy.
Introduction: One of the diseases in developed counties is myocardial infarction that causes the death of many people. A Problem of many new drugs usage concerns their assessment for applying therapeutically for heart disease. Previous studies used animal models but today many researchers tend to apply cellular models for feasibility of cellular model production and application. Methods: For this purpose, we differentiated human bone marrow mesenchymal stem cells (hBM-MDCs) into cardiomyocyte, then induced damage into cells by Isoproterenol, and finally we assayed repair of cardiomyocyte by Periostin. Damage induction and repair were confirmed by measurement of selected markers for cardiac damage and repair at mRNA level. In addition, we measured LDH activity in culture medium during damage and repair processes. Results: Our results showed LDHa and b mRNA levels increased and also cardiac markers decreased during damage of cardiomyocytes significantly. Reciprocally LDH isozymes decreased and cardiac markers increased during repair of cardiomyocytes. Discussion/conclusion: These alterations in cardiac markers after Periostin treatment demonstrate that Periostin is an effective factor on repair of cardiomyocytes. LDH activity in culture medium decreases after damage induction and increases during repair process. According to our data, Isoproterenol and Periostin are good inducer to produce damaged and repaired differentiated cardiomyocytes respectively.
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Edalati et al. (2022) studied Isoproterenol-induced ischemic damaged cardiomyocytes. Periostin was evaluated on Cardiac markers at mRNA level and LDH activity. Periostin treatment effectively repaired isoproterenol-induced damage in human bone marrow mesenchymal stem cell-derived cardiomyocytes, evidenced by increased cardiac markers and decreased LDH.
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