Genetic Manipulation of Periostin Expression in the Heart Does Not Affect Myocyte Content, Cell Cycle Activity, or Cardiac Repair
Key result
Genetic manipulation of periostin fails to stimulate cardiomyocyte DNA synthesis, mitosis, or cytokinesis.
Why the study?
Does genetic manipulation of periostin expression improve cardiac repair and cardiomyocyte regeneration in mice following myocardial infarction?
Population
Mice lacking the gene encoding periostin and mice with inducible overexpression of full-length periostin, as…
Comparison
Genetic manipulation of periostin expression in… vs Strain-matched control mice and control cell…
Design
Preclinical
Authors
Discussion
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Implication
Periostin manipulation does not promote cardiomyocyte proliferation or repair in mice; leaves open isoform- or context-specific regenerative roles pending further validation.
Structured PICO
Does genetic manipulation of periostin expression improve cardiac repair and cardiomyocyte regeneration in mice following myocardial infarction?
P
Population
Mice lacking the gene encoding periostin and mice with inducible overexpression of full-length periostin (analyzed at baseline and after myocardial infarction), as well as in vitro cell cultures.
I
Intervention
Genetic manipulation of periostin expression (knockout or inducible overexpression) in vivo, and overexpression via adenoviral vector or recombinant protein stimulation in vitro.
C
Comparator
Strain-matched control mice and control cell cultures.
O
Outcome
Cardiomyocyte DNA synthesis, mitosis, cytokinesis, heart size, cardiomyocyte number, and proliferating myocytes in the periinfarct area.surrogate
Genetic manipulation of periostin does not stimulate cardiomyocyte cell cycle reentry or myocardial regeneration following injury, challenging previous hypotheses regarding its regenerative potential.
Cite This Study
Lorts et al. (2008) studied Myocardial infarction. Genetic manipulation of periostin expression (knockout and overexpression) vs. Strain-matched controls was evaluated on Cardiomyocyte DNA synthesis, mitosis, cytokinesis, and cell cycle reentry. Genetic manipulation of periostin expression, either through gene targeting or overexpression, did not stimulate cardiomyocyte DNA synthesis, mitosis, or cytokinesis in vitro or in vivo.