Key result
A newly developed RT-nPCR system successfully identified 25 serotypes and obtained complete VP1 sequences of 23 serotypes among 858 clinical specimens positive for human enteroviruses.
Why the study?
The study was conducted to develop RT-nPCR assays for amplifying partial and complete VP1 genes of human enteroviruses from clinical samples to support etiological surveillance of enterovirus-related diseases.
Answers are grounded in this paper and cite their sources.
The newly developed RT-nPCR system provides a rapid and reliable method for molecular typing and epidemiological surveillance of human enteroviruses.
OBJECTIVE: To develop RT-nPCR assays for amplifying partial and complete VP1 genes of human enteroviruses (HEVs) from clinical samples and to contribute to etiological surveillance of HEV-related diseases. METHODS: per μL and copies per μL, and the newly established methods were tested in clinical specimens collected in recent years. RESULTS: per μL and 100 virus copies per μL, using serially-diluted virus stocks of five serotypes. As a proof-of-concept, 25 serotypes were identified and complete VP1 sequences of 23 serotypes were obtained by this system among 858 clinical specimens positive for HEVs during the past eight surveillance seasons. CONCLUSION: This RT-nPCR system is capable of amplifying the partial and complete VP1 gene of HEV A-D, providing rapid, sensitive, and reliable options for molecular typing and molecular epidemiology of HEVs in clinical specimens.
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Chen et al. (2020) studied Human enterovirus (HEV) infection (n=858). RT-nPCR assays for VP1 genes was evaluated on Identification of serotypes and complete VP1 sequences. A newly developed RT-nPCR system successfully identified 25 serotypes and obtained complete VP1 sequences of 23 serotypes among 858 clinical specimens positive for human enteroviruses.
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