Molecular Identification and Analysis of Nonserotypeable Human Enteroviruses
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Key result
A modified RT-PCR-based reverse line blot hybridization assay successfully identified 79.3% (73 of 92) of previously nonserotypeable human enterovirus clinical isolates.
Population
224 human enterovirus clinical isolates, including 132 previously studied isolates and 92 nonserotypeable…
Comparison
Modified RT-PCR-based reverse line blot… vs VP1 sequencing and conventional serotyping.
Design
Preclinical
Authors
Discussion
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Implication
May improve enterovirus typing in research labs; leaves open clinical adoption pending prospective validation.
Structured PICO
P
Population
224 human enterovirus clinical isolates, including 92 nonserotypeable isolates collected between 1979 and 2007, were analyzed to validate a modified RT-PCR-RLB assay.
I
Intervention
Modified RT-PCR-based reverse line blot (RLB) hybridization assay using two HEV-specific probes in the 5' UTR and two sets of 80 serotype-specific probes in the VP1 region.
C
Comparator
VP1 sequencing (for nonserotypeable isolates) and conventional serotyping (for previously studied isolates).
O
Outcome
Identification and genotyping of HEV isolates.
A modified RT-PCR-RLB assay using 5' UTR and VP1 probes provides a rapid and efficient method for identifying common and nonserotypeable human enteroviruses.
Limitations
- The RLB assay generally cannot distinguish sequences with a one-base difference.
- 19 nonserotypeable isolates belonging to uncommon genotypes were not identified with either set of serotype-specific probes and required VP1 sequencing.
- The assay could not detect 9 uncommon genotypes among the nonserotypeable isolates.
Cite This Study
Zhou et al. (2010) studied Human enterovirus infection (n=224). Modified RT-PCR-based reverse line blot (RLB) hybridization assay vs. VP1 sequencing and conventional serotyping was evaluated on Identification rate of nonserotypeable HEV isolates. A modified RT-PCR-based reverse line blot hybridization assay successfully identified 79.3% (73 of 92) of previously nonserotypeable human enterovirus clinical isolates.
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