Key result
A reverse transcription-PCR assay targeting the VP2 capsid protein correctly identified 61 typeable HEV isolates and matched VP1 sequencing in 87.3% of previously untypeable strains.
Population
116 clinical and environmental strains of human enterovirus (HEV)
Comparison
Reverse transcription-PCR assay targeting the… vs VP1 sequencing method and seroneutralization
Design
Other
Authors
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May facilitate culture-independent HEV typing; leaves open routine adoption pending prospective validation.
Observational (n=116)
The VP2 sequencing method is a promising and sensitive strategy for typing human enterovirus strains, potentially avoiding the need for cell culture.
Nasri et al. (2007) conducted an observational in Human enterovirus (HEV) (n=116). VP2 sequencing method vs. VP1 sequencing and seroneutralization was evaluated on Correct identification at the serotype level. A reverse transcription-PCR assay targeting the VP2 capsid protein correctly identified 61 typeable HEV isolates and matched VP1 sequencing in 87.3% of previously untypeable strains.
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