July 2, 2009Journal of Clinical Microbiology
Identification of 20 Common Human Enterovirus Serotypes by Use of a Reverse Transcription-PCR-Based Reverse Line Blot Hybridization Assay
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Population
132 human enterovirus isolates randomly selected from 6,383 HEVs isolated between 1979 and 2007 at an…
Comparison
Reverse transcription-PCR-based reverse line… vs Conventional serotyping methods and VP1 sequencing
Design
Other, 95 isolates not sequenced in VP1 were tested blinded to prior…
Authors
Discussion
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Implication
Supports RT-PCR-RLB for enterovirus genotyping; leaves open larger validation before routine diagnostic adoption.
Structured PICO
P
Population
132 human enterovirus (HEV) isolates randomly selected from 6,383 HEVs isolated between 1979 and 2007 at an Australian reference virology laboratory
I
Intervention
Reverse transcription-PCR-based reverse line blot (RT-PCR-RLB) hybridization assay
C
Comparator
Conventional serotyping methods and VP1 sequencing
O
Outcome
Concordance of RT-PCR-RLB genotypes with conventional serotypes
The RT-PCR-RLB assay is a convenient, fast, and accurate method for genotyping common human enteroviruses, complementing traditional serotyping.
Cite This Study
Zhou et al. (2009) studied this question.
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