December 16, 2005Journal of General Virology

Species-specific RT-PCR amplification of human enteroviruses: a tool for rapid species identification of uncharacterized enteroviruses

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Population

193 enterovirus prototype strains and isolates of known serotype, and 186 previously uncharacterized…

Comparison

Species-specific RT-PCR primers targeting the… vs Partial sequencing of the VP1 capsid gene

Design

Preclinical

Authors

MOM. Steven ObersteCenters for Disease Control and Prevention KMKaija MaherCenters for Disease Control and Prevention AWA. J. WilliamsCenters for Disease Control and Prevention

Discussion

Member takes

Implication

These primers may enable rapid HEV species typing in isolates; leaves open clinical adoption without human validation studies.

Structured PICO

Population

193 enterovirus prototype strains and isolates of known serotype, and 186 previously uncharacterized non-polio enterovirus isolates

Intervention

Species-specific RT-PCR primers targeting the 3'-non-translated region of enteroviruses

Comparator

Partial sequencing of the VP1 capsid gene

Outcome

Specificity and amplification rate of the species-specific RT-PCR primers

Species-specific RT-PCR primers targeting the 3'-non-translated region provide a rapid and highly specific tool for identifying human enterovirus species.

Cite This Study

Oberste et al. (2005) studied this question.

synapsesocial.com/papers/6a7c4bae24f98b6d3fc2c5df https://doi.org/10.1099/vir.0.81179-0

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Species-specific RT-PCR amplification of human enteroviruses: a tool for rapid species identification of uncharacterized enteroviruses

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Species-specific RT-PCR amplification of human enteroviruses: a tool for rapid species identification of uncharacterized enteroviruses

Discussion

Member takes

Implication

Structured PICO

Cite This Study

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Also Consider