Key result
Applied shear stress caused cultured bovine aortic endothelial cells to flatten, with maximum cell height decreasing by approximately 23% over 18 hours at 10 dyn/cm2, and become more wedge-shaped in the stream direction.
This study demonstrates a novel micro-particle tracking velocimetry methodology to quantify the three-dimensional morphological response of endothelial cells to fluid shear stress at the sub-cellular level.
Answers are grounded in this paper and cite their sources.
May enable sub-cellular shear mapping in endothelial models; leaves open in vivo validation and clinical translation.
This paper presents micro-particle tracking velocimetry measurements over cultured bovine aortic endothelial cell monolayers in microchannels. The objective was to quantify fluid forces and cell morphology at the sub-cellular scale for monolayers subjected to steady shear rates of 5, 10, and 20 dyn/cm2. The ultimate goal of this study was to develop an experimental methodology for in vitro detailed study of physiologically realistic healthy and diseased conditions. Cell topography, shear stress, and pressure distributions were calculated from sets of velocity fields made in planes parallel to the microchannel wall. For each experiment, measurements were made in 3 h intervals for 18 h. It was found that there is a three-dimensional change in cell morphology as a result of applied shear stress. That is, cells flatten and become more wedge shaped in the stream direction while conserving volume by spreading laterally, i.e., in the cross-stream direction. These changes in cell morphology are directly related to local variations in fluid loading, i.e., shear stress and pressure. This paper describes the first flow measurements over a confluent layer of endothelial cells that are spatially resolved at the sub-cellular scale with a simultaneous temporal resolution to quantify the response of cells to fluid loading.
No takes yet. Share an insight, caveat, or question.
Lambert et al. (2018) studied Endothelial cell mechanics (In vitro). Steady shear stress vs. Baseline (0th hour) was evaluated on Cell morphology (height and shape) and hemodynamic forces. Applied shear stress caused cultured bovine aortic endothelial cells to flatten, with maximum cell height decreasing by approximately 23% over 18 hours at 10 dyn/cm2, and become more wedge-shaped in the stream direction.
Synapse has enriched 10 related papers. Consider them for comparative context: