Key result
Fluid shear stress magnitude correlates with orientation time in cultured endothelial cells.
A newly designed parallel plate flow chamber successfully demonstrated that cultured endothelial cell orientation time correlates with the magnitude of applied fluid shear stress.
In vitro endothelial alignment under shear is magnitude-dependent; leaves open hemodynamic relevance to human vascular disease.
A parallel plate chamber in a flow system has been designed to study the effects of fluid shear stresses on cells. The system was applied to the study of cultured endothelial cells grown on cover slips which were accommodated in recessed wells in the base plate. Dye injection studies in the chamber indicated laminar flow over the cells. Shear rates measured over the cover slips by an electrochemical technique were found to be linear with flow rate. Laser doppler anemometry showed parabolic profiles between the plates. Endothelial cells subjected to flow showed a correlation between the time required for orientation and the magnitude of the shear stress.
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Koslow et al. (1986) studied this question. Fluid shear stress was evaluated on Correlation between time required for orientation and magnitude of shear stress. Cultured endothelial cells subjected to fluid shear stress showed a correlation between the time required for orientation and the magnitude of the shear stress.