What question did this study set out to answer?

To identify and characterize the enzymes responsible for converting inactive big endothelin-1 to mature endothelin-1 in vascular endothelial cells.

October 15, 1990FEBS Letters

Conversion of big endothelin‐1 to endothelin‐1 by two types of metalloproteinases derived from porcine aortic endothelial cells

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Population

Cultured porcine aortic endothelial cells

Design

Preclinical

Authors

YMYasuo MatsumuraAmerican Pharmacists Association RIRuriko IkegawaMitsubishi Tanabe Pharma Corporation YTYaeko TsukaharaSanten (Japan)

Discussion

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Implication

Hypothesis-generating for distinct endothelial metalloproteinases in ET-1 maturation; leaves open their relevance to human vascular disease.

Key Points

To identify and characterize the enzymes responsible for converting inactive big endothelin-1 to mature endothelin-1 in vascular endothelial cells.
Incubated big ET-1(1-39) with cytosolic and membrane fractions prepared from cultured porcine aortic endothelial cells.
Evaluated enzymatic sensitivity to metal chelators and the metalloproteinase inhibitor phosphoramidon.
Identified cleavage products using reverse-phase HPLC coupled with radioimmunoassays for endothelin and its C-terminal fragment.
Both cytosolic and membrane fractions cleaved big ET-1 between Trp21 and Val22, yielding mature ET-1(1-21) and the C-terminal fragment CTF22-39.
Metal chelators markedly inhibited conversion in both fractions, whereas phosphoramidon selectively suppressed membrane-fraction conversion without affecting cytosolic activity.

Structured PICO

Population

Cultured porcine aortic endothelial cells

Intervention

Incubation of big endothelin-1 (big ET-1(1-39)) with cytosolic or membrane fractions, and treatment with metal chelators or phosphoramidon

Outcome

Increase in immunoreactive-endothelin (IR-ET) and identification of cleavage productssurrogate

The study demonstrates the presence of two distinct metalloproteinases in vascular endothelial cells responsible for converting big endothelin-1 to active endothelin-1.

Cite This Study

Matsumura et al. (1990) studied this question.

synapsesocial.com/papers/6a7a55da2eb3c7e469f3f2b3 https://doi.org/10.1016/0014-5793(90)80475-x

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Implication

Hypothesis-generating for distinct endothelial metalloproteinases in ET-1 maturation; leaves open their relevance to human vascular disease.

Conversion of big endothelin‐1 to endothelin‐1 by two types of metalloproteinases derived from porcine aortic endothelial cells

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Conversion of big endothelin‐1 to endothelin‐1 by two types of metalloproteinases derived from porcine aortic endothelial cells

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider