What question did this study set out to answer?

To evaluate whether cardiac ankyrin repeat protein (CARP) serves as a sensitive genetic marker of cardiac hypertrophy in vivo and in vitro, and to determine its upstream transcriptional regulatory mechanisms.

July 1, 2000HypertensionOpen Access

Cardiac Ankyrin Repeat Protein Is a Novel Marker of Cardiac Hypertrophy

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Authors

YAYasushi AiharaBunkyo University MKMasahiko KurabayashiElectrophysiology YSYuichiro SaitoGunma University Hospital

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Implication

CARP may mark experimental hypertrophy; leaves open translation to human hypertension or clinical use.

Key Points

To evaluate whether cardiac ankyrin repeat protein (CARP) serves as a sensitive genetic marker of cardiac hypertrophy in vivo and in vitro, and to determine its upstream transcriptional regulatory mechanisms.
Measured CARP expression across three rat models of cardiac hypertrophy: abdominal aortic constriction, spontaneously hypertensive rats, and Dahl salt-sensitive rats.
Performed transient transfection assays in primary neonatal rat cardiac myocytes overexpressing stress-activated MAPK pathway components p38 and Rac1.
Characterized transcriptional activation using promoter mutation analysis and electrophoretic mobility shift assays (EMSA).
CARP expression was markedly increased across all three rat hypertrophy models and correlated strongly with brain natriuretic peptide mRNA levels in Dahl rats.
Overexpression of p38 and Rac1 directly activated transcription from both CARP and brain natriuretic peptide promoters in neonatal cardiac myocytes.
Mutation and binding assays identified the M-CAT element as the essential binding site mediating CARP promoter induction in response to stress-responsive MAPK signaling.

Cite This Study

Aihara et al. (2000) studied this question.

synapsesocial.com/papers/6a7b1bf75968508e56f3f136 https://doi.org/10.1161/01.hyp.36.1.48

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Cardiac Ankyrin Repeat Protein Is a Novel Marker of Cardiac Hypertrophy

Discussion

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Implication

Key Points

Cite This Study

Cardiac Ankyrin Repeat Protein Is a Novel Marker of Cardiac Hypertrophy

Discussion

Member takes

Implication

Key Points

Cite This Study

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