What question did this study set out to answer?

To investigate whether cardiac ankyrin repeat protein (CARP) expression correlates with asymmetric postnatal ventricular growth and responds to doxorubicin-induced cardiomyopathy in vivo.

February 21, 2004European Journal of Heart Failure

Left–Right Asymmetric Ventricular Expression of CARP in the Piglet Heart: Regional Response to Experimental Heart Failure

View Full Paper

Ask AI

Bookmark

Population

Piglet model of early postnatal development and doxorubicin-induced cardiomyopathy (Dox-CM)

Comparison

Doxorubicin (Dox) treatment vs Untreated/normal postnatal development

Design

Preclinical

Authors

MTMario TorradoUniversidade da Coruña ELEduardo LópezCalifornia State University, Monterey Bay ACAlberto CentenoUniversidade da Coruña

Discussion

Member takes

Implication

CARP asymmetry marks early LV maturation but vanishes in Dox-CM; hypothesis-generating for ventricular pathways in pediatric cardiomyopathy.

Key Points

To investigate whether cardiac ankyrin repeat protein (CARP) expression correlates with asymmetric postnatal ventricular growth and responds to doxorubicin-induced cardiomyopathy in vivo.
Quantified CARP mRNA and protein levels in piglet left and right ventricles during early postnatal development and following doxorubicin-induced cardiomyopathy.
Analyzed myocardial tissue samples using Northern blot hybridization, semi-quantitative RT-PCR, and Western blot assays.
CARP mRNA and protein levels were significantly higher in the left ventricle than the right ventricle throughout normal early postnatal development.
Doxorubicin treatment caused dose-dependent and chamber-dependent accumulation of CARP mRNA and protein in the ventricular myocardium.
Progression of doxorubicin-induced cardiomyopathy to overt heart failure completely abolished the baseline left-versus-right asymmetry of ventricular CARP expression.

Structured PICO

Population

Piglet model of early postnatal development and doxorubicin-induced cardiomyopathy (Dox-CM)

Intervention

Doxorubicin (Dox) treatment

Comparator

Untreated/normal postnatal development

Outcome

CARP mRNA and protein levels in the left and right ventriclessurrogate

CARP expression is differentially regulated in the left and right ventricles during postnatal development, and its expression pattern changes during the transition to doxorubicin-induced heart failure in a piglet model.

Cite This Study

Torrado et al. (2004) studied this question.

synapsesocial.com/papers/6a76e91e6789de8d0af6e488 https://doi.org/10.1016/j.ejheart.2003.11.004

Topics

Heart failure HFrEF treatment

View Full Paper

Ask AI

Bookmark

Implication

CARP asymmetry marks early LV maturation but vanishes in Dox-CM; hypothesis-generating for ventricular pathways in pediatric cardiomyopathy.

Left–Right Asymmetric Ventricular Expression of CARP in the Piglet Heart: Regional Response to Experimental Heart Failure

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Topics

Left–Right Asymmetric Ventricular Expression of CARP in the Piglet Heart: Regional Response to Experimental Heart Failure

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Topics

Also Consider