What question did this study set out to answer?

To determine how non-structural proteins of cytopathogenic and non-cytopathogenic bovine viral diarrhea virus biotypes regulate host cell apoptosis and beta interferon promoter activation.

September 30, 2009Journal of General Virology

Expression of the NS3 protease of cytopathogenic bovine viral diarrhea virus results in the induction of apoptosis but does not block activation of the beta interferon promoter

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Authors

TGT GamlenUniversity of Oxford KRKathryn H. RichardsUniversity of Leeds JMJamel MankouriUniversity of Leeds

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Implication

Experimental study reveals that BVDV NS3 protease triggers intrinsic apoptosis without inhibiting the interferon-beta promoter in cultured cells, highlighting mechanisms of viral pathogenesis.

Key Points

To determine how non-structural proteins of cytopathogenic and non-cytopathogenic bovine viral diarrhea virus biotypes regulate host cell apoptosis and beta interferon promoter activation.
Expressed NS3 or NS2-3 alongside cofactor NS4A from cytopathogenic and non-cytopathogenic BVDV strains in cultured cells.
Assessed activation of caspase-9 and caspase-3 using wild-type and protease-inactive mutant NS3/4A constructs.
Measured TLR3- and RIG-I-dependent activation of the beta interferon promoter in the presence of BVDV proteases.
Expression of NS3/4A elevated caspase-9 and caspase-3 activity to trigger intrinsic apoptosis, whereas protease-inactive NS3/4A failed to induce apoptosis.
BVDV proteases did not block TLR3- or RIG-I-dependent activation of the beta interferon promoter, displaying a distinct regulatory profile from related viruses.

Cite This Study

Gamlen et al. (2009) studied this question.

synapsesocial.com/papers/6a7c00441f8f1eb7b8ed4e29 https://doi.org/10.1099/vir.0.016170-0

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Implication

Experimental study reveals that BVDV NS3 protease triggers intrinsic apoptosis without inhibiting the interferon-beta promoter in cultured cells, highlighting mechanisms of viral pathogenesis.

Expression of the NS3 protease of cytopathogenic bovine viral diarrhea virus results in the induction of apoptosis but does not block activation of the beta interferon promoter

Discussion

Member takes

Implication

Key Points

Cite This Study

Expression of the NS3 protease of cytopathogenic bovine viral diarrhea virus results in the induction of apoptosis but does not block activation of the beta interferon promoter

Discussion

Member takes

Implication

Key Points

Cite This Study

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