November 1, 1999Journal of VirologyOpen Access

Assignment of the Multifunctional NS3 Protein of Bovine Viral Diarrhea Virus during RNA Replication: an In Vivo and In Vitro Study

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Population

Autonomous subgenomic bovine viral diarrhea virus (BVDV) RNA replicon (DI9c) and purified mutant NS3 proteins

Comparison

In-frame mutations in the NS3 coding unit vs Wild-type BVDV RNA replicon

Design

Preclinical

Authors

CGClaus W. GrassmannJustus-Liebig-Universität Gießen OIOlaf IskenInstitute of Virology of the Slovak Academy of Sciences SBSven‐Erik BehrensLuther University

Discussion

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Implication

May guide NS3-targeted antivirals; leaves open translation from animal models to clinical efficacy.

Structured PICO

Population

Autonomous subgenomic bovine viral diarrhea virus (BVDV) RNA replicon (DI9c) and purified mutant NS3 proteins

Intervention

In-frame mutations in the NS3 coding unit (including replacement of the third position of the DEYH helicase motif II by T or F, and a 4-amino acid insertion in the C-terminal part)

Comparator

Wild-type BVDV RNA replicon

Outcome

RNA replication capability, polyprotein proteolysis, and ATPase/RNA helicase activitysurrogate

The diverse enzymatic activities of the BVDV NS3 protein, particularly the ATPase/RNA helicase, play a pivotal role in early steps of viral replication.

Cite This Study

Grassmann et al. (1999) studied this question.

synapsesocial.com/papers/6a82f4bf3b194ef92e581bcf https://doi.org/10.1128/jvi.73.11.9196-9205.1999

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Assignment of the Multifunctional NS3 Protein of Bovine Viral Diarrhea Virus during RNA Replication: an In Vivo and In Vitro Study

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Assignment of the Multifunctional NS3 Protein of Bovine Viral Diarrhea Virus during RNA Replication: an In Vivo and In Vitro Study

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider