September 14, 2004Journal of VirologyOpen Access

Temporal Modulation of an Autoprotease Is Crucial for Replication and Pathogenicity of an RNA Virus

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Population

Madin-Darby bovine kidney cells, BHK-21 cells, and bovine viral diarrhea virus strains CP7 and NCP7

Comparison

Mutagenesis of the NS2 autoprotease vs Wild-type BVDV strains

Design

Preclinical

Follow-up

Up to 72 hours post-electroporation/infection

Authors

TLT. LacknerJustus-Liebig-Universität Gießen AMAnja MüllerJustus-Liebig-Universität Gießen APAlexander PankrazBioScientia (Poland)

Discussion

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Implication

NS2 autoprotease is essential for BVDV replication; leaves open targeted antiviral development beyond animal models.

Structured PICO

Population

Madin-Darby bovine kidney (MDBK) cells, BHK-21 cells, and bovine viral diarrhea virus (BVDV) strains CP7 (cytopathogenic) and NCP7 (noncytopathogenic)

Intervention

Mutagenesis of the NS2 autoprotease (including catalytic residues His1447 and Cys1512)

Comparator

Wild-type BVDV strains

Outcome

NS2-3 cleavage efficiency and viral RNA replication (measured by NS3 expression and virus progeny titer)surrogate

The NS2 autoprotease is essential for BVDV RNA replication, and its temporal modulation plays a crucial role in viral pathogenicity.

Cite This Study

Lackner et al. (2004) studied this question.

synapsesocial.com/papers/6a8344406bd0b01a4e8cef83 https://doi.org/10.1128/jvi.78.19.10765-10775.2004

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Temporal Modulation of an Autoprotease Is Crucial for Replication and Pathogenicity of an RNA Virus

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Temporal Modulation of an Autoprotease Is Crucial for Replication and Pathogenicity of an RNA Virus

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider