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June 1, 1991Journal of Biological ChemistryOpen Access

Expression of a constitutively activated mutant of the beta-isozyme of protein kinase C in cardiac myocytes stimulates the promoter of the beta-myosin heavy chain isogene

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Population

Cultured neonatal rat cardiac myocytes

Comparison

Co-transfection with expression plasmids… vs Co-transfection with wild-type alpha-PKC…

Design

Preclinical

Authors

KKK. KariyaMassachusetts General HospitalLKLarry R. KarnsUniversity of VirginiaPSPaul SimpsonUniversity of North Carolina at Chapel Hill

Discussion

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Implication

Animal myocyte data indicate beta-PKC preference for beta-MHC; leaves open isozyme-selective targeting in human cardiac disease.

Structured PICO

P
Population
Cultured neonatal rat cardiac myocytes
I
Intervention
Co-transfection with expression plasmids encoding constitutively activated mutants of the beta-isozyme of protein kinase C (PKC)
C
Comparator
Co-transfection with wild-type alpha-PKC, wild-type beta-PKC, or constitutively activated alpha-PKC mutant
O
Outcome
Stimulation of the beta-MHC or alpha-MHC promoterssurrogate

Transcription of the beta-MHC isogene is preferentially stimulated by beta-PKC in cardiac myocytes, demonstrating differential functions of PKC isozymes in transcriptional regulation.

Cite This Study

Kariya et al. (1991) studied this question.

synapsesocial.com/papers/6a7c0ee57cc40aa1f8dfa562https://doi.org/10.1016/s0021-9258(18)99178-7
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Differential activation of protein kinase C alpha is associated with arachidonate release in Madin-Darby canine kidney cells.1990 · 86 citations
  2. 2Alpha 1-adrenergic receptor stimulation of sarcomeric actin isogene transcription in hypertrophy of cultured rat heart muscle cells.1989 · 137 citations
  3. 3Thyroid hormone regulates expression of a transfected alpha-myosin heavy-chain fusion gene in fetal heart cells.1987 · 76 citations
  4. 4Differential regulation of skeletal alpha-actin transcription in cardiac muscle by two fibroblast growth factors.1990 · 104 citations
  5. 5Firefly luciferase gene: structure and expression in mammalian cells.1987 · 3,093 citations