August 1, 1998Journal of Biological ChemistryOpen Access

Translation Initiation of a Cardiac Voltage-gated Potassium Channel by Internal Ribosome Entry

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Population

Transiently transfected mammalian cells and in vitro translation assay using extracts from…

Comparison

Bicistronic vector containing the 5'-UTR of… vs Control construct

Design

Preclinical

Authors

DNDeborah NegulescuUniversity of California, Irvine LLLouis E.-C. LeongFaraday Technology (United States)KCK. George ChandyNanyang Technological University

Discussion

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Implication

Preclinical data with no immediate clinical implications; leaves open whether IRES regulates Kv1.4 expression in human cardiomyocytes.

Structured PICO

Population

Transiently transfected mammalian cells and in vitro translation assay using extracts from poliovirus-infected HeLa cells to test the 5'-UTR of mouse Kv1.4 mRNA

Intervention

Bicistronic vector containing the 5'-UTR of mouse Kv1.4 mRNA in the intercistronic region between two reporter genes (chloramphenicol acetyltransferase and luciferase)

Comparator

Control construct (bicistronic vector without the mKv1.4 UTR)

Outcome

Translation of the downstream reporter gene (luciferase)surrogate

The 5'-untranslated region of the mammalian Kv1.4 voltage-gated potassium channel mRNA contains a functional internal ribosome entry segment that allows for cap-independent translation.

Cite This Study

Negulescu et al. (1998) studied this question.

synapsesocial.com/papers/6a7c113ba540d02696c2bf9e https://doi.org/10.1074/jbc.273.32.20109

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Translation Initiation of a Cardiac Voltage-gated Potassium Channel by Internal Ribosome Entry

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Translation Initiation of a Cardiac Voltage-gated Potassium Channel by Internal Ribosome Entry

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider