What does this research mean for the field?

Product-based, Vmax-oriented kinetic analysis using multi-concentration assessment provides a more reliable evaluation of bacterial bile salt hydrolase (BSH) activity than substrate depletion methods, which fail to distinguish between experimental groups. Novelty: ClaimNovelty.METHODOLOGICAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

What question did this study set out to answer?

To assess bile salt hydrolase activity reliably and characteristically using a new LC-MS-based assay.

August 12, 2026Scientific ReportsOpen Access

Product-based kinetic analysis reveals limitations of substrate depletion method for assessing bile salt hydrolase activity

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Authors

MUMartin UherArizona State University HLHana LaštůvkováUniversity Hospital Hradec Králové LPLinda PetráčkováCharles University

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Implication

Randomized trial evaluates bile salt hydrolase activity using a new assay in cholestasis models, highlighting analysis methods.

Key Points

To assess bile salt hydrolase activity reliably and characteristically using a new LC-MS-based assay.
Developed an LC-MS-based assay to measure BSH activity in murine cecal content.
Evaluated BSH kinetics using tauro-conjugated substrates in a cholestasis model induced by DDC.
Conducted multi-concentration assessments to determine enzyme activity.
Apparent K m values indicated decreasing substrate affinity in the order: TDCA < TCDCA < TCA.
BSH activity in DDC-treated mice showed a significant reduction with decreased apparent V max.
Substrate depletion approaches did not consistently differentiate between experimental groups.

Cite This Study

Uher et al. (2026) studied this question.

synapsesocial.com/papers/6a7c20ae06a85aed514b7b1e https://doi.org/10.1038/s41598-026-66161-5

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Product-based kinetic analysis reveals limitations of substrate depletion method for assessing bile salt hydrolase activity

Discussion

Member takes

Implication

Key Points

Cite This Study

Product-based kinetic analysis reveals limitations of substrate depletion method for assessing bile salt hydrolase activity

Discussion

Member takes

Implication

Key Points

Cite This Study

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