What question did this study set out to answer?

This research aims to understand how bile salt hydrolase homologues influence bile resistance in Lactobacillus johnsonii.

April 1, 2026Journal of Agricultural and Food Chemistry

Two Bile Salt Hydrolase Homologues Drive Bile Resistance in Lactobacillus johnsonii ATCC 43121

View Full Paper

Ask AI

Bookmark

Authors

HWHua WuInstitute of Food Science & Technology YCYujie ChenZhejiang Normal University JHJing-Wei HuInstitute of Food Science & Technology

Discussion

Member takes

Implication

Functional analysis reveals that bile salt hydrolase homologues improve bile resistance in Lactobacillus johnsonii, suggesting their vital role in intestinal adaptation.

Key Points

This research aims to understand how bile salt hydrolase homologues influence bile resistance in Lactobacillus johnsonii.
Conducted knockout and complementation experiments on three BSH homologues (BSHA, BSHB, BSHC)
Performed functional assays under simulated human intestinal conditions
Used molecular docking and dynamics simulations to analyze enzyme interactions
BSHA and BSHC significantly improved bile resistance, while BSHB was ineffective due to low activity
BSHA and BSHC hydrolyzed conjugated bile acids efficiently, preserving cellular conditions
Molecular docking indicated structural hindrance in BSHB's active site, limiting its function

Cite This Study

Wu et al. (2026) studied this question.

synapsesocial.com/papers/69ccb5f716edfba7beb87bb7 https://doi.org/10.1021/acs.jafc.5c14654

View Full Paper

Ask AI

Bookmark

Implication

Functional analysis reveals that bile salt hydrolase homologues improve bile resistance in Lactobacillus johnsonii, suggesting their vital role in intestinal adaptation.

Two Bile Salt Hydrolase Homologues Drive Bile Resistance in Lactobacillus johnsonii ATCC 43121

Discussion

Member takes

Implication

Key Points

Cite This Study

Two Bile Salt Hydrolase Homologues Drive Bile Resistance in Lactobacillus johnsonii ATCC 43121

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider