What question did this study set out to answer?

Investigate the subtype-specific role of bile salt hydrolase (BSH) in Lactobacillus strains and characterize how BSH1 regulates bile acid metabolism to alleviate intrahepatic cholestasis.

September 17, 2026npj Biofilms and MicrobiomesOpen Access

From gene to function: unraveling the BSH1-mediated mechanism of Lactobacillus in alleviating intrahepatic cholestasis

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Authors

YLYaru LiuJiangnan University LYLeilei YuJiangnan University CZChuan ZhangJiangnan University

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Implication

Preclinical study demonstrates that BSH1-producing Lactobacillus strains alleviate liver injury in cholestatic mice, suggesting a targeted microbial approach to treat intrahepatic cholestasis.

Key Points

Investigate the subtype-specific role of bile salt hydrolase (BSH) in Lactobacillus strains and characterize how BSH1 regulates bile acid metabolism to alleviate intrahepatic cholestasis.
Genotyped BSH-harboring Lactobacillus strains using comparative genomics and measured enzymatic activity across subtypes via in vitro bile salt co-culture assays.
Administered wild-type and BSH1-knockout Lactiplantibacillus plantarum CCFM242 to cholestatic mice to assess liver biochemistry, gut microbiota, and hepatic/ileal bile acid transporters.
Profiled fecal metagenomes from patients with intrahepatic cholestasis of pregnancy and healthy controls to evaluate clinical BSH subtype distribution.
BSH catalytic activity was driven by enzyme genotype rather than gene copy number, with BSH1-encoding strains displaying the highest activity.
BSH1-positive strains significantly decreased serum liver enzymes and total bile acids to attenuate hepatic injury in mice, whereas BSH1 knockout completely eliminated therapeutic efficacy.
BSH1 shifted gut microbiota composition, modulated the FXR–FGF15 axis, upregulated hepatic BSEP to increase bile excretion, and downregulated ileal ASBT to decrease reabsorption, mirroring findings in healthy human controls.

Cite This Study

Liu et al. (2026) studied this question.

synapsesocial.com/papers/6aabb7975f706d05830e6b76 https://doi.org/10.1038/s41522-026-01156-8

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Implication

Preclinical study demonstrates that BSH1-producing Lactobacillus strains alleviate liver injury in cholestatic mice, suggesting a targeted microbial approach to treat intrahepatic cholestasis.

From gene to function: unraveling the BSH1-mediated mechanism of Lactobacillus in alleviating intrahepatic cholestasis

Discussion

Member takes

Implication

Key Points

Cite This Study

From gene to function: unraveling the BSH1-mediated mechanism of Lactobacillus in alleviating intrahepatic cholestasis

Discussion

Member takes

Implication

Key Points

Cite This Study

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