Synapse
⌘+K
Synapse
PulseExploreClubsResearchersJournals
Instagram
HomeClubsExplore
August 1, 1995Journal of VirologyOpen Access

Identification of the active-site residues of the L proteinase of foot-and-mouth disease virus

View Full Paper
Ask AI
Bookmark
Share

Population

Foot-and-mouth disease virus (FMDV) leader (L) protein, cell-free system, and transfected cells

Comparison

Specific mutations introduced into the L gene vs Wild-type FMDV L protein

Design

Preclinical

Authors

MPM E PicconeMZMarla ZellnerTKThomas F. Kumosinski

Discussion

Loading...

Member takes

Overview

Cys-23/His-120 mutations identify FMDV Lpro targets; leaves open antiviral translation beyond animal models.

Structured PICO

P
Population
Foot-and-mouth disease virus (FMDV) leader (L) protein, cell-free system, and transfected cells
I
Intervention
Specific mutations introduced into the L gene (Cys-23 to Ala, His-120 to Leu, Cys-23 to Ser, His-110 to Leu)
C
Comparator
Wild-type FMDV L protein
O
Outcome
Autocatalytic cleavage at the L/P1 junction and cleavage of translation initiation factor p220surrogate

Identifies key active-site residues (Cys-23 and His-120) essential for the proteolytic activity of the FMDV L proteinase.

Cite This Study

Piccone et al. (1995) studied this question.

synapsesocial.com/papers/6a7c29e305df6d7f2ea1595ehttps://doi.org/10.1128/jvi.69.8.4950-4956.1995
View Full Paper
Ask AI
Bookmark
Share

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Proteolytic processing of foot-and-mouth disease virus polyproteins expressed in a cell-free system from clone-derived transcripts1987 · 106 citations
  2. 2Foot-and-mouth disease virus leader proteinase: purification of the Lb form and determination of its cleavage site on eIF-4 gamma1994 · 193 citations
  3. 3Antiviral effects of a thiol protease inhibitor on foot-and-mouth disease virus1992 · 75 citations
  4. 4Capsid intermediates assembled in a foot-and-mouth disease virus genome RNA-programmed cell-free translation system and in infected cells1985 · 66 citations
  5. 5RGD sequence of foot-and-mouth disease virus isessential for infecting cells via the natural receptor but can be bypassed by anantibody-dependent enhancement pathway.1994 · 254 citations