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July 22, 2016MicrocirculationOpen Access

Multiple Endo-PAT measurements within the same day significantly increased the reactive hyperemia index by 30.1% at 4 hours compared to baseline, demonstrating poor intraday repeatability.

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Why the study?

Does the timing of Endo-PAT measurements affect the repeatability of Reactive Hyperemia Index (RHI) in healthy volunteers?

Population

Healthy volunteers (31 male subjects for interday repeatability, 16 volunteers for intraday repeatability)

Design

Cross-sectional

Follow-up

Up to 2 consecutive days

Key result

Multiple Endo-PAT measurements within the same day significantly increased the reactive hyperemia index by 30.1% at 4 hours compared to baseline, demonstrating poor intraday repeatability.

Authors

CBCristian Del Bo’VDValeria DeonMPMarisa Porrini

Discussion

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Member takes

Overview

May caution against repeated same-day Endo-PAT testing; supports protocol standardization in microvascular research.

Study Design

Type

Observational (n=47)

Multicenter

No

Structured PICO

Does the timing of Endo-PAT measurements affect the repeatability of Reactive Hyperemia Index (RHI) in healthy volunteers?

P
Population
47 healthy male volunteers aged 20 to 30 years underwent Endo-PAT measurements to assess the intra- and interday repeatability of peripheral arterial function.
E
Exposure
Reactive Hyperemia Index (RHI) measurement by Endo-PAT
O
Outcome
Inter- and intraday repeatability of RHIsurrogate

Main Result

p-value: p=<0.001

Endo-PAT measurements of RHI show good interday but poor intraday repeatability, highlighting the need to standardize protocols and avoid multiple same-day measurements.

Limitations

  • Small sample size
  • Study population restricted to healthy young males, limiting generalizability
  • Potential confounding effect of light breakfast, although a fasting subgroup confirmed the time-dependent RHI intraday variability

Cite This Study

Bo’ et al. (2016) conducted an observational in Healthy (n=47). Multiple intraday Endo-PAT measurements vs. Baseline measurement was evaluated on Intraday repeatability of reactive hyperemia index (RHI) (p=<0.001). Multiple Endo-PAT measurements within the same day significantly increased the reactive hyperemia index by 30.1% at 4 hours compared to baseline, demonstrating poor intraday repeatability.

synapsesocial.com/papers/6a7c5905d501feb4353bee6ehttps://doi.org/10.1111/micc.12300
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