September 1, 2019Veterinary WorldOpen Access

Modification on acute myocardial infarction model through left anterior descending coronary artery ligation: An experimental study on rats (Rattus norvegicus) using readily available materials

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Why the study?

Creating animal models for MI is hindered by low survival rates, complicated techniques, complexities confirming MI incidence, and requirements for complex surgical tools.

Does a modified left anterior descending coronary artery ligation technique without tracheal incision and ventilator induce a consistent acute myocardial infarction model in Wistar rats?

Population

48 Wistar rats

Comparison

Coronary artery ligation group vs sham group

Design

Experimental animal study

Follow-up

6 weeks

Authors

JNJohanes NugrohoAirlangga University WYWiwik Misaco YuniartiAirlangga University AWArdyan WardhanaUniversity of Surabaya

Discussion

Member takes

Implication

Simplifies acute MI induction in rats with basic tools; leaves open translation to clinical research.

Structured PICO

Does a modified left anterior descending coronary artery ligation technique without tracheal incision and ventilator induce a consistent acute myocardial infarction model in Wistar rats?

Population

48 14-week-old male Wistar rats, weighing an average of 200-300 g.

Intervention

Left anterior descending (LAD) coronary artery ligation modification technique performed 1-2 mm distal to the left atrial appendage, without tracheal incision and ventilator.

Comparator

Sham group (thoracotomy without LAD coronary artery ligation).

Outcome

Myocardial infarction occurrence evaluated using heart enzyme parameters (SGOT, CKMB) 24 h post-ligation and histological studies of the infarcted area 6 weeks after the ligation.surrogate

A modified LAD coronary artery ligation technique without a ventilator or tracheal incision successfully induced a consistent myocardial infarction model in rats, with a 50% survival rate at 24 hours and no long-term mortality up to 6 weeks.

Limitations

Did not perform imaging studies such as echocardiography or gadolinium-enhanced magnetic resonance imaging to select rats with consistent infarct sizes.
Did not evaluate if the deaths within the first 24 h post-MI were due to surgical errors or very large infarct sizes.
Did not perform specific pharmacological intervention that would validate this model.
Did not perform ECG assessment, apply oxygen saturation and end-tidal carbon dioxide, and control the temperature during the anesthetic procedure.

Cite This Study

Nugroho et al. (2019) studied this question.

synapsesocial.com/papers/6a7cb724178e986690cf8768 https://doi.org/10.14202/vetworld.2019.1448-1453

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Modification on acute myocardial infarction model through left anterior descending coronary artery ligation: An experimental study on rats (Rattus norvegicus) using readily available materials

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

Modification on acute myocardial infarction model through left anterior descending coronary artery ligation: An experimental study on rats (Rattus norvegicus) using readily available materials

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

Also Consider

Also Consider