Characterization of the target DNA sequence for the DNA-binding domain of zinc finger protein 191

Studies on the DNA-binding properties of transcription factors are important in searching for the downstream genes regulated by these factors. In the present study, we report on the DNA-binding property of a Cys2His2-type transcription factor, zinc finger protein 191 (Zfp191), which has been newly found to play a significant role in mice. By constructing a fusion protein containing the DNA-binding domain of Zfp191, we characterized target DNA by determining the protein's binding specificity and dependence on zinc. The data showed that the DNA-binding domain of Zfp191 can specifically bind to the TCAT repeat motif and that there is a cooperative effect among the target DNA's multiple binding sites. Furthermore, the binding reaction is dependent on zinc. This work provides a foundation for further studies on the role of Zfp191 in gene regulation and development.