piR-45962 exacerbates autophagic apoptosis in aged myocardial ischemia-reperfusion injury through modulation of the HNRNPH1/GATA5 signaling axis

, and the indicators of apoptosis and autophagy are significantly different. Mechanistic research of piR-45962 shows that heterogeneous nuclear ribonucleoprotein 1 (HNRNPH1) is a target protein for piR-45962, and as an RNA-binding protein implicated in apoptotic regulation, HNRNPH1 could interact with GATA binding protein 5 (GATA5) to exacerbate autophagic apoptosis. Together, our results reveal the mechanism by which piR-45962 downregulates GATA5 expression through upregulation of HNRNPH1, thereby establishing a novel piR-45962-HNRNPH1-GATA5 regulatory axis that contributes to aged myocardial injury during I/R.