May 1, 1983Journal of Lipid ResearchOpen Access

Retention of gangliosides in serum delipidated by diisopropyl ether-1-butanol extraction.

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Population

Serum samples (endogenous serum gangliosides and radiolabeled rat brain gangliosides added to serum)

Design

Preclinical

Authors

CWCH WongClaremont Graduate University SLStephan LadischChildren's National

Discussion

Member takes

Implication

Diisopropyl ether-1-butanol extraction cannot remove gangliosides from serum or plasma; leaves open need for validated alternative depletion methods in lipid research.

Structured PICO

Population

Serum samples (endogenous serum gangliosides and radiolabeled rat brain gangliosides added to serum)

Intervention

Extraction with diisopropyl ether-1-butanol

Outcome

Retention of gangliosides in the aqueous phasesurrogate

Diisopropyl ether-1-butanol extraction cannot be used to remove gangliosides from serum or plasma, as they are retained in the aqueous phase.

Cite This Study

Wong et al. (1983) studied this question.

synapsesocial.com/papers/6a7d0de453702837bd9ffa10 https://doi.org/10.1016/s0022-2275(20)37974-8

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Implication

Diisopropyl ether-1-butanol extraction cannot remove gangliosides from serum or plasma; leaves open need for validated alternative depletion methods in lipid research.

Retention of gangliosides in serum delipidated by diisopropyl ether-1-butanol extraction.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Retention of gangliosides in serum delipidated by diisopropyl ether-1-butanol extraction.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider