What question did this study set out to answer?

To describe the mechanism and structural consequences of the calcium paradox phenomenon triggered by sequential calcium-free and calcium-containing perfusion in isolated hearts.

January 1, 2000Cardiovascular ResearchOpen Access

Morphological changes of heart muscle caused by successive perfusion with calcium-free and calcium-containing solutions (calcium paradox)

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Population

Isolated rat hearts perfused according to the Langendorff technique

Design

Review

Authors

WHW.C. HülsmannErasmus University Rotterdam

Discussion

Member takes

Implication

May caution against calcium-free solutions in myocardial preservation; leaves open optimal reintroduction strategies for clinical translation.

Key Points

To describe the mechanism and structural consequences of the calcium paradox phenomenon triggered by sequential calcium-free and calcium-containing perfusion in isolated hearts.
Perfused isolated rat hearts via the Langendorff technique using calcium-containing Ringer solution followed by calcium-free 0.15 M aqueous sodium acetylacetate.
Reintroduced calcium-containing Ringer solution after transient calcium depletion to monitor cardiac electromechanical function and myocardial morphology.
Calcium-free perfusion induced electromechanical dissociation, while subsequent calcium reintroduction caused irreversible systolic arrest, myoglobin leakage, and alternating hypercontracted and empty sarcomeres.
Decalcification of the plasmalemma increased phospholipid membrane mobility, creating severe membrane permeability upon recalcification that can be attenuated by hypothermia or amphipathic compounds.

Structured PICO

Population

Isolated rat hearts perfused according to the Langendorff technique

Intervention

Successive perfusion with calcium-free solution followed by calcium-containing solution

Outcome

Morphological changes (hypercontracted cardiomyocytes, loss of myoglobin, tissue destruction)surrogate

The 'calcium paradox' phenomenon highlights that calcium-free perfusion followed by calcium reintroduction causes severe myocardial tissue destruction, serving as a critical warning for cardiac surgery.

Cite This Study

W.C. Hülsmann (2000) studied this question.

synapsesocial.com/papers/6a7d1b7a2c71273e4183d788 https://doi.org/10.1016/s0008-6363(99)00303-x

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Implication

May caution against calcium-free solutions in myocardial preservation; leaves open optimal reintroduction strategies for clinical translation.

Morphological changes of heart muscle caused by successive perfusion with calcium-free and calcium-containing solutions (calcium paradox)

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Morphological changes of heart muscle caused by successive perfusion with calcium-free and calcium-containing solutions (calcium paradox)

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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