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July 1, 2004Journal of Biological ChemistryOpen Access

Paradoxical Actions of Endogenous and Exogenous Insulin-like Growth Factor-binding Protein-5 Revealed by RNA Interference Analysis

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Population

Human osteosarcoma cells

Comparison

IGFBP-5 siRNA and/or addition of exogenous IGFBP-5 vs Control cells, wild type IGFBP-5 overexpression…

Design

Preclinical

Authors

PYPing YinQXQijin XuCDCunming Duan

Discussion

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Overview

Preliminary cell findings leave IGFBP-5 targeting open; in vivo validation required before any translational consideration.

Structured PICO

P
Population
Human osteosarcoma cells
I
Intervention
IGFBP-5 siRNA (knock-down of endogenous IGFBP-5) and/or addition of exogenous IGFBP-5
C
Comparator
Control cells, wild type IGFBP-5 overexpression, or IGFBP-4 overexpression
O
Outcome
Cell survival/apoptosis (measured by TUNEL-positive cells) and bone differentiation (alkaline phosphatase activity)surrogate

Endogenous and exogenous IGFBP-5 exhibit opposing biological actions on cell survival in human osteosarcoma cells, highlighting the importance of loss-of-function approaches.

Cite This Study

Yin et al. (2004) studied this question.

synapsesocial.com/papers/6a7d4f4bb62e30e3311cc976https://doi.org/10.1074/jbc.m401378200
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