June 15, 1992FEBS LettersOpen Access

The identification and characterisation of an actin‐binding site in α‐actinin by mutagenesis

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Population

E. coli expressing the N-terminal actin-binding domain of alpha-actinin as a fusion protein with…

Design

Preclinical

Authors

PKPhilip A. KuhlmanUniversity of Leicester LHLance HemmingsUniversity of Leicester DCDavid R. CritchleyUniversity of Leicester

Discussion

Member takes

Implication

Maps alpha-actinin actin-binding in vitro; leaves open relevance to cardiac sarcomere function.

Structured PICO

Population

E. coli expressing the N-terminal actin-binding domain of alpha-actinin as a fusion protein with glutathione-S-transferase

Intervention

N- and C-terminal deletions and amino acid substitutions within the actin-binding domain

Outcome

Identification of the actin-binding site and characterization of the interaction with F-actin

The study identifies residues 120-134 as the actin-binding site in alpha-actinin and demonstrates that its interaction with F-actin is predominantly hydrophobic.

Cite This Study

Kuhlman et al. (1992) studied this question.

synapsesocial.com/papers/6a7da253cf7e8b32dacb4e4c https://doi.org/10.1016/0014-5793(92)80619-r

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The identification and characterisation of an actin‐binding site in α‐actinin by mutagenesis

Discussion

Member takes

Implication

Structured PICO

Cite This Study

The identification and characterisation of an actin‐binding site in α‐actinin by mutagenesis

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider