January 1, 1973Proceedings of the National Academy of SciencesOpen Access

Double-Stranded RNA as an Inhibitor of Protein Synthesis and as a Substrate for a Nuclease in Extracts of Krebs II Ascites Cells

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Population

Extracts of Krebs II ascites cells (S-30 extracts)

Comparison

Double-stranded RNA from various sources (P… vs Control incubations without dsRNA or with…

Design

Preclinical

Authors

HRHugh D. RobertsonThe Honourable Society of Lincoln's Inn MMMichael B. MathewsRutgers, The State University of New Jersey

Discussion

Member takes

Implication

Hypothesis-generating for dsRNA effects on translation; leaves open relevance to cardiovascular RNA models.

Structured PICO

Population

Extracts of Krebs II ascites cells (S-30 extracts)

Intervention

Double-stranded RNA (dsRNA) from various sources (P. chrysogenum, reovirus, phage f2, poly(G-C))

Comparator

Control incubations without dsRNA or with single-stranded RNA

Outcome

Rate of protein synthesis (translation of EMC viral RNA and mouse globin mRNA) measured by incorporation of 14C-labeled amino acidssurrogate

Double-stranded RNA inhibits protein synthesis in Krebs II ascites cell extracts, which also contain a dsRNA-digesting nuclease that reduces their sensitivity to dsRNA compared to reticulocyte lysates.

Cite This Study

Robertson et al. (1973) studied this question.

synapsesocial.com/papers/6a7dc30087be3f923b5ca1d2 https://doi.org/10.1073/pnas.70.1.225

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January 1, 1973Proceedings of the National Academy of SciencesOpen Access

Double-Stranded RNA as an Inhibitor of Protein Synthesis and as a Substrate for a Nuclease in Extracts of Krebs II Ascites Cells

View Full Paper

Ask AI

Bookmark

Population

Extracts of Krebs II ascites cells (S-30 extracts)

Comparison

Double-stranded RNA from various sources (P… vs Control incubations without dsRNA or with…

Design

Preclinical

Authors

HRHugh D. RobertsonThe Honourable Society of Lincoln's Inn MMMichael B. MathewsRutgers, The State University of New Jersey

Discussion

Member takes

Implication

Hypothesis-generating for dsRNA effects on translation; leaves open relevance to cardiovascular RNA models.

Structured PICO

Population

Extracts of Krebs II ascites cells (S-30 extracts)

Intervention

Double-stranded RNA (dsRNA) from various sources (P. chrysogenum, reovirus, phage f2, poly(G-C))

Comparator

Control incubations without dsRNA or with single-stranded RNA

Outcome

Rate of protein synthesis (translation of EMC viral RNA and mouse globin mRNA) measured by incorporation of 14C-labeled amino acidssurrogate

Cite This Study

Robertson et al. (1973) studied this question.

synapsesocial.com/papers/6a7dc30087be3f923b5ca1d2 https://doi.org/10.1073/pnas.70.1.225

Also Consider

Synapse has enriched 3 closely related papers on similar clinical questions. Consider them for comparative context:

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