What question did this study set out to answer?

To directly evaluate whether locally produced prostaglandins mediate renin secretion triggered by macula densa sensing of tubular salt concentrations.

October 1, 1993AJP Renal Physiology

Effect of prostaglandin synthesis inhibition on macula densa-stimulated renin secretion

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Why the study?

Does prostaglandin synthesis inhibition reduce macula densa-stimulated renin secretion in rabbit kidney juxtaglomerular apparatus specimens?

Population

Individual juxtaglomerular apparatus specimens microdissected from rabbit kidney

Comparison

Cyclooxygenase inhibitors flurbiprofen (10 M) or… vs Control specimens without cyclooxygenase…

Design

Preclinical

Authors

SGSuzanne GreenbergMichigan Department of Health and Human Services JLJ. LorenzUniversität Hamburg XHXiaoxin HeFujian Medical University

Discussion

Member takes

Implication

Supports prostaglandin mediation of macula densa renin secretion in rabbit JGA; leaves open translation to human physiology or clinical practice.

Key Points

To directly evaluate whether locally produced prostaglandins mediate renin secretion triggered by macula densa sensing of tubular salt concentrations.
Microdissected individual juxtaglomerular apparatus specimens from rabbit kidneys.
Perfused specimens with high-NaCl (141 meq/l Na+, 122 meq/l Cl-) or low-NaCl (26 meq/l Na+, 7 meq/l Cl-) solutions.
Assessed renin secretion responses during step changes in NaCl concentration in the presence or absence of cyclooxygenase inhibitors (10^-5 M flurbiprofen or 10^-4 M flufenamic acid).
A step decrease in perfusate NaCl significantly increased renin secretion rate from 15.06 to 63.1 nGU/min (P < 0.01) in a reversible manner.
Treatment with cyclooxygenase inhibitors abolished low-NaCl-induced renin secretion (flurbiprofen: 3.5 to 10.5 nGU/min, not significant; flufenamic acid: 9.0 to 12.3 nGU/min, not significant).
A step increase in perfusate NaCl reversibly suppressed renin secretion in controls, but suppression was absent in specimens treated with flufenamic acid.

Structured PICO

Does prostaglandin synthesis inhibition reduce macula densa-stimulated renin secretion in rabbit kidney juxtaglomerular apparatus specimens?

Population

Individual juxtaglomerular apparatus specimens microdissected from rabbit kidney

Intervention

Cyclooxygenase inhibitors flurbiprofen (10(-5) M) or flufenamic acid (10(-4) M) added to bathing solution during step changes in perfusate NaCl concentration

Comparator

Control specimens without cyclooxygenase inhibitors exposed to the same step changes in perfusate NaCl concentration

Outcome

Renin secretion ratesurrogate

Locally produced prostaglandins may act as a primary mediator of the renin response to macula densa activation.

Cite This Study

Greenberg et al. (1993) studied this question.

synapsesocial.com/papers/6a7dd3102d182a3d7e99aa11 https://doi.org/10.1152/ajprenal.1993.265.4.f578

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Implication

Supports prostaglandin mediation of macula densa renin secretion in rabbit JGA; leaves open translation to human physiology or clinical practice.

Effect of prostaglandin synthesis inhibition on macula densa-stimulated renin secretion

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Effect of prostaglandin synthesis inhibition on macula densa-stimulated renin secretion

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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