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December 15, 1993Proceedings of the National Academy of SciencesOpen Access

The min K channel underlies the cardiac potassium current IKs and mediates species-specific responses to protein kinase C.

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Population

Xenopus oocytes expressing cloned guinea pig (gp) min K potassium channel

Comparison

Stimulation with protein kinase A and protein… vs Unstimulated oocytes or wild-type gpmin K channels

Design

Preclinical

Authors

MVMichael D. VarnumWashington State UniversityABAE BuschOregon Health & Science UniversityCBChris T. BondNeoleukin Therapeutics (United States)

Discussion

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Implication

Species-specific PKC responses in guinea pig IKs caution against human extrapolation; leaves open N102's role in arrhythmia models.

Structured PICO

P
Population
Xenopus oocytes expressing cloned guinea pig (gp) min K potassium channel
I
Intervention
Stimulation with protein kinase A (CPT-cAMP, isoproterenol) and protein kinase C (phorbol 12,13-didecanoate), and site-directed mutagenesis (N102S)
C
Comparator
Unstimulated oocytes or wild-type gpmin K channels
O
Outcome
Electrophysiological properties (current amplitude, voltage dependence, kinetics) of the expressed potassium currents (gpIsK)surrogate

The guinea pig min K protein underlies the slow component of the delayed rectifier potassium current (IKs) in ventricular myocytes, and its N102 residue mediates species-specific responses to PKC.

Cite This Study

Varnum et al. (1993) studied this question.

synapsesocial.com/papers/6a7e1401fc4f0e65bd3bfa7dhttps://doi.org/10.1073/pnas.90.24.11528
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Calcium-sensitive delayed rectifier potassium current in guinea pig ventricular cells1990 · 149 citations
  2. 2Intracellular Ca2+ and protein kinase C modulate K+ current in guinea pig heart cells1987 · 138 citations
  3. 3Cloning of a Membrane Protein That Induces a Slow Voltage-Gated Potassium Current1988 · 523 citations
  4. 4Cloning and expression of the delayed-rectifier IsK channel from neonatal rat heart and diethylstilbestrol-primed rat uterus.1990 · 190 citations
  5. 5Effects of the New Class III Antiarrhythmic Drug E-4031 on Myocardial Contractility and Electrophysiological Parameters1991 · 91 citations