Population
Rabbit skeletal muscle F-actin and F-actin in lysates of rabbit polymorphonuclear leukocytes (PMNs)
Design
Preclinical
Authors
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DNase I may enable TRITC-phalloidin quantification of F-actin in lysates; leaves open validation in cardiovascular cell models.
TRITC-phalloidin can be used to measure F-actin levels in cell lysates without prior fixation if DNase I is included to prevent phalloidin-induced polymerization.
Cano et al. (1992) studied this question.
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