October 1, 1995Journal of VirologyOpen Access

Temperature-sensitive hepatitis A virus mutants with deletions downstream of the first pyrimidine-rich tract of the 5' nontranslated RNA are impaired in RNA synthesis

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Population

Cultured BS-C-1 cells infected with Hepatitis A virus mutants

Comparison

Temperature shifts and specific deletions in the… vs Standard 5'NTR or non-ts revertant

Design

Preclinical

Authors

Discussion

No immediate implications for HAV management; leaves open targeting of this 5'NTR segment for antivirals or attenuated vaccines.

Population

Cultured BS-C-1 cells infected with Hepatitis A virus (HAV) mutants (specifically temperature-sensitive mutant delta 131-144 and delta 116-144)

Intervention

Temperature shifts (31 degrees C vs 37 degrees C) and specific deletions in the 5' nontranslated RNA (5'NTR)

Comparator

Standard 5'NTR or non-ts revertant

Outcome

Viral replication and RNA synthesissurrogate

The single-stranded RNA segment containing nt 140 to 144 in the 5'NTR of Hepatitis A virus plays a critical role in viral RNA synthesis.

Shaffer et al. (1995) studied this question.

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