What question did this study set out to answer?

To determine whether renal ischemia and reperfusion induce the conversion of xanthine dehydrogenase to xanthine oxidase, generating toxic oxygen metabolites that impair post-ischemic renal recovery.

March 1, 1990AJP Renal Physiology

Role of xanthine oxidase in ischemia/reperfusion injury

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Authors

SLStuart L. LinasDenver Health Medical Center DWD. WhittenburgUniversity of Colorado Hospital JRJohn E. RepineUniversity of Colorado Denver

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Implication

Experimental study demonstrates that ischemia-reperfusion drives xanthine oxidase conversion in rat kidneys, indicating that enzyme depletion protects against reperfusion injury.

Key Points

To determine whether renal ischemia and reperfusion induce the conversion of xanthine dehydrogenase to xanthine oxidase, generating toxic oxygen metabolites that impair post-ischemic renal recovery.
Measured type D (dehydrogenase) and type O (oxidase) xanthine oxidase activities in rat renal cortical homogenates following 30 minutes of in vivo ischemia and 60 minutes of isolated perfused kidney reperfusion.
Depleted renal xanthine oxidase in rats via a 4-week tungsten diet to evaluate its direct role in functional renal recovery during reperfusion.
Ischemia increased total type O activity (53 ± 5% vs. 21 ± 3%, P < 0.01) and reversible type O activity (15.4 ± 1.5 vs. 2.1 ± 1.4 U/g) relative to nonischemic controls, while total enzyme activity remained unchanged.
Reperfusion further escalated total type O activity to 82 ± 3% and reversible type O activity to 27.7 ± 3.3 U/g (both P < 0.01 vs. nonischemic perfusions).
Tungsten-mediated depletion reduced renal xanthine oxidase levels by >90% and markedly improved renal function during subsequent reperfusion.

Cite This Study

Linas et al. (1990) studied this question.

synapsesocial.com/papers/6a7ec4dad93698698fb99240 https://doi.org/10.1152/ajprenal.1990.258.3.f711

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Implication

Experimental study demonstrates that ischemia-reperfusion drives xanthine oxidase conversion in rat kidneys, indicating that enzyme depletion protects against reperfusion injury.

Role of xanthine oxidase in ischemia/reperfusion injury

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Implication

Key Points

Cite This Study

Role of xanthine oxidase in ischemia/reperfusion injury

Discussion

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Implication

Key Points

Cite This Study

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