Key result
Methionine restriction prevents weight gain but fails to improve blood pressure or arterial reactivity.
Why the study?
High-fat diets reportedly cause loss of anti-contractile properties in perivascular adipose tissue, prompting investigation into whether dietary methionine restriction prevents this dysfunction by increasing ATF4 expression.
Does dietary methionine restriction prevent high-fat diet-induced PVAT dysfunction in male Wistar rats?
Does dietary methionine restriction prevent high-fat diet-induced PVAT dysfunction in male Wistar rats?
In male Wistar rats, 12 weeks of dietary methionine restriction prevents body mass gain but does not alter blood pressure or arterial reactivity.
Does not support methionine restriction for vascular protection in this rat model; leaves open effects on high-fat diet-induced PVAT dysfunction.
Perivascular adipose tissue (PVAT) regulates vascular tone, and high-fat diets reportedly lead to the loss of its anti-contractile properties. Methionine restriction recapitulates many caloric restriction metabolic effects and increases liver expression of activating transcription factor 4 (ATF4) and downstream targets with anti-contractile properties such as cystathionine-β-synthase (CBS) and cystathionine-γ-lyase (CSE). We hypothesized that dietary methionine restriction would prevent high-fat diet-induced PVAT dysfunction by increasing ATF4 expression. Male Wistar rats were fed control (C, 10% calories from fat) or high-fat diets (H, 60% calories from fat) (HFD) that were methionine replete (R, 0.86% methionine) or deplete (D, 0.12% methionine) for 12 weeks. Methionine restriction prevented body mass increases, independent of fat content, and had no effect on blood pressure or arterial contraction to norepinephrine (10 μM) or relaxation to acetylcholine (10 μM). In this feeding paradigm, HFD did not induce PVAT dysfunction. CR diet increased ATF4 transcript levels in multiple PVAT depots but did not consistently affect expression of downstream targets. Protein expression of ATF4 and its targets varied largely for all groups. In conclusion, 12 week methionine restriction profoundly affected body mass but not blood pressure or arterial reactivity in the presence or absence of PVAT.
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A 2025 study studied High-fat diet-induced PVAT dysfunction. Dietary methionine restriction vs. Methionine replete (0.86% methionine) was evaluated on Body mass, blood pressure, and arterial reactivity. Dietary methionine restriction for 12 weeks prevented body mass increases but had no effect on blood pressure or arterial reactivity in male Wistar rats.
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