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March 4, 2025The FASEB JournalOpen Access

High-fat, high-sugar, high-salt diet drives metabolic syndrome and sex-specific vascular changes in mice.

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Why the study?

Perivascular adipose tissue expansion promotes inflammation and vascular dysfunction in metabolic syndrome, but the sexual dimorphisms of perivascular adipose tissue are poorly understood.

Does a high-fat, high-sugar, high-salt diet induce sex-specific changes in aortic function and inflammation in a mouse model of metabolic syndrome?

Population

Six-week-old male and female C57BL/6 mice

Comparison

High-fat, high-sugar, and high-salt diet vs normal chow diet

Design

Preclinical animal study

Follow-up

10 weeks

Key result

A high-fat, high-sugar, and high-salt diet induced metabolic syndrome and caused contrasting effects on vascular function with and without perivascular adipose tissue between male and female mice.

Authors

VTVivian TranHBHolly BrettleHDHenry Diep

Discussion

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Member takes

Overview

May suggest sex-specific vascular responses in metabolic syndrome models; leaves open human translation and clinical relevance.

Structured PICO

Does a high-fat, high-sugar, high-salt diet induce sex-specific changes in aortic function and inflammation in a mouse model of metabolic syndrome?

P
Population
Six-week-old male and female C57BL/6 mice
I
Intervention
High-fat diet (43% kcal in food) with high sugar and salt in drinking water (10% high fructose corn syrup and 0.9% NaCl; HFSS) for 10 weeks
C
Comparator
Normal chow diet (NCD) for 10 weeks
O
Outcome
Aortic function and inflammation characterized at endpoint using pin myography, flow cytometry, bulk RNA-sequencing, GSEA analysis, and histologysurrogate

Main Result

p-value: p=<0.05

A novel diet-induced metabolic syndrome mouse model reveals significant sexual dimorphism in how perivascular adipose tissue regulates vascular function.

Cite This Study

Tran et al. (2025) studied Metabolic syndrome. High-fat diet with high sugar and salt (HFSS) vs. Normal chow diet (NCD) was evaluated on Weight gain, fasting blood glucose, systolic blood pressure, aortic fibrosis, and perivascular adipocyte cross-sectional area (p=<0.05). A high-fat, high-sugar, and high-salt diet induced metabolic syndrome and caused contrasting effects on vascular function with and without perivascular adipose tissue between male and female mice.

synapsesocial.com/papers/6a125c4331014d4abe342b3dhttps://doi.org/10.1096/fj.202401871r
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