The Role of ANGPTL Gene Family Members in Hepatocellular Carcinoma

Figures

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  Figure 1. The mRNA expression levels of ANGPTL1 in several common cancer cell lines from the CCLE database.

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  Figure 2. Relative expression of ANGPTLs in HCC samples and normal tissue samples from the UCSC database. Violin plot shows the expression of ANGPTL1 (a) and ANGPTL3 (b) mRNAs in HCC samples relative to normal samples, based on the TCGA database. LIHC: abbreviation for HCC of the liver from the TCGA database.

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  Figure 3. Relative expression of ANGPTLs in HCC samples, normal tissue, and cirrhotic and healthy samples in the HCCDB database. mRNA expression levels of ANGPTL1 (a) and ANGPTL8 (b) in different HCC datasets in the HCCDB database. Red: HCC samples; blue: adjacent normal tissue samples; cyan: cirrhotic samples; orange: healthy samples.

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  Figure 4. Comparison of protein levels of ANGPTL1 (a), ANGPTL2 (b), ANGPTL3 (c), ANGPTL4 (d), and ANGPTL5 (e) in HCC tissues with those of normal tissues by immunohistochemical staining.

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  Figure 5. Correlation of ANGPTL7 (a) and ANGPTL8 (b) with gender based on TCGA database analysis.

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  Figure 6. Association of expression of ANGPTLs with patient prognosis. The Kaplan-Meier plotter database was used to assess the correlation between the expression of ANGPTL1 (a), ANGPTL2 (b), ANGPTL3 (c), and ANGPTL4 (d) and the prognosis of HCC patients.

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  Figure 7. Gene mutation and biological interaction network of ANGPTLs in HCC. (a) Frequency and type of gene mutation of ANGPTLs in HCC patients from the TCGA database were analyzed by the cBioPortal database. Different colors show different types of gene alterations. (b) Interacting gene networks of ANGPTL gene families in HCC were collected through the STRING database. The red color represents seed genes and the blue color represents neighboring genes.

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  Figure 8. KEGG pathway enrichment analysis of ANGPTL1 coexpressed genes in HCC and miRNA targets of ANGPTL1. (a) Volcano plot showing differential expression of genes associated with ANGPTL1 in HCC and correlation analysis using Pearson. Green: negatively associated significant genes; red: positively associated significant genes. (b) Heat map showing the top 50 positively associated and top 50 negatively associated significant genes of ANGPTL1. (c) Analysis of KEGG pathway enrichment of ANGPTL1 coexpressed genes in HCC based on gene set enrichment analysis (GSEA). (d) miRNA of ANGPTL1 in HCC targets.